ANTIBODY DEVELOPEMENT - HISTOLOGY TOOLS - ANTIBODIES
HomeHistology ProductsAntibody ServicesAntibodies
2100 Retriever
Retriever Manual
Ordering Retriever
IHC Detection Systems

2100 Retriever


It is easy to process sections with Retriever. First you load the slides into Slide Rack.


6 Slide Racks (108 slides) can be placed in Retriever at once. This means you can use up to 6 different buffers at once.



And you push the Start button (OK, you will have to close the lid first).

The solution for Antigen Unmasking

Our Unique Retriever solves the problem of staining formalin fixed tissues. This is an affordable solution to all known major problems with immunohistochemistry on paraffin sections. Ease of use combined with high reproducibility of the results will give you the best quality immunostaining. The Retriever is a bench-top model for thermally processing slides of formalin-fixed, paraffin embedded tissues prior to immunostaining.

The model has been designed to ensure identical processing of all the samples during a processing cycle, as well as the identical processing of the samples in individual sessions. The Retriever preserves processed tissues.

Now you can:

  • Run antigen unmasking in 6 various buffers at once.
  • Perform gentle antigen retrieval that does not damage the tissue morphology.
  • Get identical results every time.
  • Compare series of slides treated in independent sessions.


How does the Retriever work?

Ok, basically it is a pressure cooker. However, a pressure cooker specially designed to unmask the antigen on tissue sections. The core principle is heating of the chambers with the slides at high temperature(>120°C) and high pressure. Sensors control the heating profile for the temperature and pressure to be reached at certain pace and over certain time. We did a lot of tests to find the optimal settings. When the required temperature is reached, it will be kept for several minutes. After that the slides will be cooling over 2 hours. Specially designed thermal walls of the unit control the speed of cooling of the inner chamber and slides.

Who would benefit from using our Retriever?

  • Investigative Pathology, where the high quality of staining (a picture may be published!) is required
  • Any lab that is short on technical personnel: any student or post-doc can process slides for the staining without using much time
  • Small routine pathology labs, where a limited number of slides should be processed daily
  • Anyone who uses highly valuable samples, such as tissue arrays or unique tissue samples
  • Simplicity and reliability of the unit ensures the safety of your sample, and a high quality antigen unmasking.

How To Use:
To read more about Retriever, please download the Retriever Manual me publications about it.



Retriever protects tissue morphology
This is a section of intestinal tissue fixed with formalin and embedded into paraffin Processing the tissue in microwave-type machines results in damage of the morphology of the gentle tissues. No damage to morphology occurs, however, after processing the tissue in Retriever, although the unmasking is usually better than after the microwave

Examples of staining after processing the tissue sections in 2100 Retriever

Ki-67 (antibody MIB1)
Routine proliferation marker detected on formalin-fixed paraffin embedded section of human intestine. Please note the strong nuclear staining, extremely clean background (no non-specific binding of the antibody) and excellent morphology of the tissue including the preservation of the mucosal secret in the cells and undamaged membranes. Buffer A was used for processing.






To order Retriever>>>

CD8 (monoclonal antibody)
Infiltrating cytotoxic CD8T- cells were detected in formalin fixed, paraffin embedded section of human intestine after antigen unmasking in Retriever. Buffer B was used for processing.


 





To order Retriever>>>

PCNA (monoclonal antibody)
Marker for proliferating cells is shown for cells of human intestine. Note the morphology of the tissue that is completely unchanged by processing.






To order Retriever>>>

HomeHistology ProductsAntibody ServicesAntibodies