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	Epitope Advice

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	Mighty Rabbit Protocol

	Hybridoma Services

	Hybridomas for licensing

	Ordering the Services

Ordering the Services

To order our services please choose one of the options below, depending on the status of your project and your requirements in detailed discussion. When the details of your project are established, we will send you a quotation which will also include a standard discount for your next project. Customers with several antigens are advised to contact our sales for special conditions (we have groups that developed 20-50 individual antibodies using Express Rabbit).

I would like to inquire about new Express Rabbit project
Please ask for quotation

I need to select a peptide epitope from my protein sequence
Please submit the sequence in one-letter code or provide an accession number

I want to ship antigen, how much or antigen and where to ship
Please download the instructions

I have already an antigen, I want to place an order for the protocol
Please fill the form

I want to discuss a new hybridoma project
Please inquire

What is the pricing for Hybridoma project?
See this page

Rabbit Express Protocol Pricing (Prices are valid from May 1st, 2010)
Code	Service	Description	Price
S0561	Pre-immune serum collection	Price per protocol, 2-4 ml of serum is collected before immunization at day 0	35.-
S0562	Pre-immune screening bleeds	Collection of pre-immune serum and maintenance of the rabbit for 7 days while customer analyzes the serum (price per rabbit)	39.-
S0500	Express Protocol, Rabbit, Standard (28 days)	1 Rabbit protocol, complete, including adjuvant, all immunizations, immunomodulators and bleeds. 2 bleeds at day 21 and 28 are collected (approximately 15-20 and 50-60 ml of serum respectively). The last bleed is an exsang bleed.	425.-
S0510	Express Protocol, Standard, for 2 Rabbits	As S0500, but 2 rabbits are injected with the same antigen	798.- (399.- per rabbit)
S0501	Express Protocol, Rabbit, Extended (49 days)	1 Rabbit protocol, complete, including adjuvant, all immunizations, immunomodulators and bleeds. 2 bleeds at day 21 and 28 are collected. Additional boost is given at day 28, 2 more bleeds are collected (day 42 and final bleed at day 49). Rabbit is terminated at day 49.	599.-
S0511	Express Protocol, Extended, for 2 Rabbits (49 days)	As S0501, but 2 rabbits are injected with the same antigen	1158.-
Additional Operations
S0540	Rabbit, Extra Injections	Includes all injections performed at one time (i.e. one subcutaneous and one intramuscular)	21.-
S0550	Rabbit, Extended Maintenance	Price per animal per day	2.90
S0560	Bleed, Rabbit	Regular bleed, 15-20 ml of serum	35.-
S0563	Aliquoting serum	Serum is usually supplied as one 5, 20 or 80 ml vial. Serum may be aliquoted into smaller volumes according to the customer requirements. Price is per every extra vial	5.-
S0580	Affinity purification. small scale	Affinity purification of specific immunoglobulins from 15-20 ml of serum. Performed on a column with antigen attached.	315.-
S0590	Affinity purification large scale	Affinity purification of specific immunoglobulins from 25 to 50 ml of serum. Performed on a column with antigen attached.	395.-
S0591	Affinity purification large scale	Affinity purification of specific immunoglobulins from 51 to 75 ml of serum. Performed on a column with antigen attached.	495.-
S0592	Isolation of IgG fraction from serum	Protein A/G chromatography, per mg isolated	12.50
S0593	Lyophilization	Freeze-drying of affinity purified antibodies	inquire
Important Information
All prices are in EURO (applicable to all European/European Union countries or in U$ Dollars (all other countries) The prices do not include shipping to a customer and packaging (where applicable) The prices do not include local tax (or VAT) where applicable. All shipments to customers are done by FedEx. Some countries are served by DHL or WorldCorier

Basic Hybridoma Protocols
Protocol	Standard	Extended
Advice on Peptide Epitope	Optional	Included
Peptide Synthesis (<16 aa)	Optional	Included
Peptide Synthesis (16-30 aa)	Optional	Optional
Phosphorylation/Acetylation	Optional	Optional
Other Peptide Modifications**	Optional	Optional
Conjugation to a Carrier Protein*	Included	Included
Standard Adjuvant	Included	Included
Number of Mice	4	8
Strain of Mice	Balb/c or F1(CBC6)	Balb/c or F1(CBC6)
Fusions	as required	as required
Screening on 1 antigen***	Included	Included
Isotype-specific screening	Optional	Optional
Test-Bleeding	2	as required
Number of clones	up to 15.000	up to 20.000
Number of Guaranteed primary clones	5	10
Number of clones re-cloned 2 times****	2	3
Storage of your cells for 6 month	Included	Included
Ordering Codes	S001	S002
Price of Basic Protocol (USD or EURO)	From 4,425.-	From 6,895.-
* The most optimal carrier as discussed with the customer (KLH, Ovalbumin, etc.) See move available options at peptide extra services * If you would like the fusion being tested against a number of antigens, please inquire **** Unless agreed otherwise, we isolate all primary clones. Out of those 5 or 10 go for immediate re-cloning, the rest are frozen. Recloning of additional clones is an extra service.